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http://cio.repositorioinstitucional.mx/jspui/handle/1002/380| FABRICATION OF FLUORESCENT NANOSTRUCTURED ORGANIC MATERIALS AND THEIR APPLICATION AS CONTRAST AGENTS IN CONFOCAL AND TWO-PHOTON MICROSCOPY | |
| LAURA APARICIO IXTA | |
| Gabriel Ramos-Ortiz | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| FLUORESCENCE, ORGANIC MATERIALS, ORGANIC NANOSTRUCTURED MATERIALS, TWO-PHOTON MICROSCOPY, NANOPARTICLES, PHOTOLUMINESCENT, ABSORPTION | |
| "During the past years, technological advances have provided new uses of fluorescent materials to impact in fields such as optoelectronics, information technology and, more recently, nanotechnology. One of these technologies is fluorescence microscopy which is one of the most versatile imaging techniques in biomedical research. Particularly, twophoton laser scanning microscopy (TPM) has attracted increasing attention due to several advantages over traditional techniques such as higher resolution, higher depth penetration in tissue, reduction of photobleaching and weak autofluorescence. These advantages allow to study in detail not only cellular or tissue structure, but also get information about cellular dynamics (biochemical and biophysical processes), generate medical imaging for diagnostic purposes, and monitor the site-specific delivery of a drug into a bio-specimen. To implement TPM, the biological sample under study is stained with fluorescent markers (contrast agents). Nevertheless, it should be pointed out that the commercially available organic fluorescent materials (fluorophores) currently used in two-photon imaging exhibit very small twophoton absorption (TPA) with cross-sections commonly in the range of 1–100 GM (1 GM = 10-50 cm -4s -1). This means an area of opportunity to develop new contrast agents to provide higher sensitivity in TPM. Intensive investigations during the last two decades have produced several reports on organic materials possessing much higher TPA properties than those commercially available; however, the toxicity of the organic solvents in which they are soluble, the decreased fluorescence properties exhibited in aqueous media and the difficult processability of these materials to fabricate nanostructures to improve their solubility in water are some factors that limit their applications in the two-photon imaging technique. In this dissertation, novel organic compounds synthesized to achieve optimized fluorescent and TPA properties are studied and their processing into aqueous suspension of nanostructures is developed. Accordingly, this work focuses in two aspects, one related with basic research and other with practical application of the material under study. For the former, the (molecular) structure / (optical) property relationship is obtained through spectroscopy techniques, while for the latter the bioimaging of cellular lines of biomedical interest is pursued." | |
| 2016-02 | |
| Tesis de doctorado | |
| Inglés | |
| León, Guanajuato | |
| Público en general | |
| Aparicio Ixta, (2016). "Fabrication of fluorescent nanostructured organic materials and their application as contrast agents in confocal and two-photon microscopy". Tesis de Doctorado en Ciencias (Óptica). Centro de Investigaciones en Óptica, A.C. León, Guanajuato. 113 pp. | |
| ÓPTICA | |
| Aparece en las colecciones: | DOCTORADO EN CIENCIAS (ÓPTICA) |
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