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http://cio.repositorioinstitucional.mx/jspui/handle/1002/1362| Lensless microscopy using partially coherent synthetic light sources: Effects on the resolution of the reconstructed object. | |
| MIGUEL ANGEL ORJUELA MORENO | |
| Geminiano Martinez-Ponce | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| Spatial coherence Diffraction Autofocusing metrics | |
| "This research addresses limitations in lensless holographic microscopy with highly coherent sources, particularly speckle noise. We propose and validate a Schell-Gaussian illumination source with adjustable spatial coherence to optimize system resolution and robustness. The illumination source was implemented and characterized using rotating diffuser disks, demonstrating that rotation velocity effectively suppresses speckle by transforming the intensity distribution from exponential to Gaussian - indicating improved uniformity. Constant interference fringe visibility in Young’s experiment confirmed characteristic Schell-Gauss behavior regardless of slit displacement. Evaluation of autofocus metrics identified Intensity Variance and Local Contrast as most precise, exhibiting sharper peaks and lower uncertainty with reduced coherence. Speckle suppression significantly improved resolution, decreasing the limit from 22.09 μm to 17.59 μm, and further to 9.8 μm (from 11.0 μm) using a high-resolution sensor. The system demonstrated multi-plane focusing capability and effective operation with biological samples. This study validates spatial coherence control as fundamental for optimizing resolution and image quality in lensless digital holographic microscopy, establishing foundations for designing accessible high-performance systems." | |
| 22-08-2025 | |
| Tesis de maestría | |
| Inglés | |
| Orjuela Moreno, M. A. (2025). Lensless microscopy using partially coherent synthetic light sources: Effects on the resolution of the reconstructed object. [Tesis de maestría. Centro de Investigaciones en Óptica, A. C.] | |
| HOLOGRAFÍA | |
| Versión aceptada | |
| acceptedVersion - Versión aceptada | |
| Aparece en las colecciones: | MAESTRIA EN CIENCIAS (ÓPTICA) |
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| Fichero | Descripción | Tamaño | Formato | |
|---|---|---|---|---|
| 18574.pdf | Texto completo/ Full-text | 1.5 MB | Adobe PDF | Visualizar/Abrir |