Por favor, use este identificador para citar o enlazar este ítem: http://cio.repositorioinstitucional.mx/jspui/handle/1002/472
Optimizing the Fluorescent Yield in Two-Photon Laser Scanning Microscopy with Dispersion Compensation
RAMON CARRILES JAIMES
Acceso Abierto
Atribución-NoComercial-SinDerivadas
A challenge for nonlinear imaging in living tissue is to maximize the total fluorescent yield from each fluorophore. We investigated the emission rates of three fluorophores—rhodamine B, a red fluorescent protein, and CdSe quantum dots—while manipulating the phase of the laser excitation pulse at the focus. In all cases a transform-limited pulse maximized the total yield to insure the highest signal-to-noise ratio. Further, we find evidence of fluorescence antibleaching in quantum dot samples
21-06-2010
Artículo
CIENCIAS FÍSICO MATEMÁTICAS Y CIENCIAS DE LA TIERRA
Aparece en las colecciones: Articulos Arbitrados 2010

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