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http://cio.repositorioinstitucional.mx/jspui/handle/1002/472
Optimizing the Fluorescent Yield in Two-Photon Laser Scanning Microscopy with Dispersion Compensation | |
RAMON CARRILES JAIMES | |
Acceso Abierto | |
Atribución-NoComercial-SinDerivadas | |
A challenge for nonlinear imaging in living tissue is to maximize the total fluorescent yield from each fluorophore. We investigated the emission rates of three fluorophores—rhodamine B, a red fluorescent protein, and CdSe quantum dots—while manipulating the phase of the laser excitation pulse at the focus. In all cases a transform-limited pulse maximized the total yield to insure the highest signal-to-noise ratio. Further, we find evidence of fluorescence antibleaching in quantum dot samples | |
21-06-2010 | |
Artículo | |
CIENCIAS FÍSICO MATEMÁTICAS Y CIENCIAS DE LA TIERRA | |
Aparece en las colecciones: | Articulos Arbitrados 2010 |
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Optimizing the fluorescent yield in two-photon.pdf | 1.42 MB | Adobe PDF | Visualizar/Abrir |