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http://cio.repositorioinstitucional.mx/jspui/handle/1002/426| PHOTOCHEMICAL SYNTHESIS OF HEPARIN-BASED GOLD NANOPARTICLES AND THEIR USE IN SURFACE ENHANCED RAMAN SPECTROSCOPY | |
| María del Pilar Rodríguez-Torres | |
| Luis Armando Diaz-Torres | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| UV-VIS SPECTROSCOPY, SCANNING ELECTRON MICROSCOPY, NANOPARTICLES, RAMAN SPECTROSCOPY | |
| "This dissertation deals with gold nanoparticle green synthesis in aqueous solution using heparin, as a reducing and stabilizing agent, without other reagents through photochemistry, and their usage as surface enhanced Raman colloidal substrates. This work is divided in four parts: The first one is about proposing the photochemical synthesis method used to prepare the heparinbased gold nanoparticles in aqueous solution using UVA light (λ=366 nm). Two types of experiments were carried out: The nanoparticles were synthesized using reactive and pharmaceutical-grade heparins. Nanoparticle characterization was carried out with UV-Vis spectroscopy and Scanning Electron Microscopy. In the second one, a nanoparticle formation mechanism is proposed with the aid of UV-Vis and FTIR spectroscopies and pH measurements. In the third part, surface enhanced Raman activity is investigated, finding that reactive-grade heparin gold nanoparticles yield good Raman signals and the pharmaceutical-grade ones do not. The probe molecules used were differently charged dyes: Methylene Blue (-), Rosebengal (+) and Neutral Red (+-); as well as proteins (GP120 and Bovine Serum Albumin). There are no reports on heparin-gold nanoparticles being used for this purpose to our knowledge. In the final section, this dissertation´s contributions are presented as a closure to the research that was performed." | |
| 2015-09 | |
| Tesis de doctorado | |
| Inglés | |
| León, Guanajuato | |
| Público en general | |
| Rodríguez-Torres (2015). "Photochemical synthesis of heparin-based gold nanoparticles and their use in Surface Enhanced Raman Spectroscopy". Tesis de Doctorado en Ciencias (Óptica). Centro de Investigaciones en Óptica, A.C. León, Guanajuato. 107 pp. | |
| ÓPTICA | |
| Aparece en las colecciones: | DOCTORADO EN CIENCIAS (ÓPTICA) |
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