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http://cio.repositorioinstitucional.mx/jspui/handle/1002/1140| Polarization microscopy with stellated gold nanoparticles for robust, in-situ monitoring of biomolecules | |
| Elder de la Rosa Cruz | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| Advances in plasmonic nanoparticle synthesis afford new opportunities for biosensing applications. Here, we apply a combination of a new type of plasmonic nanomaterial – stellated nanoparticles, and polarization-sensitive darkfield microscopy for detecting molecular assemblies and tracking of individual epidermal growth factor receptors within single live cells with high signal-to-background ratio. Depolarization of linear polarized light by stellated nanoparticles is over 15-fold more efficient than similarly-sized spheroidal nanoparticles. This efficient light depolarization allows robust detection of molecules labeled with stellated nanoparticles in cross-polarized imaging where the intrinsic light scattering from cells is significantly reduced. The imaging can be carried out with single molecule sensitivity for essentially unlimited time with no signal degradation. | |
| 31-01-2008 | |
| Artículo | |
| CIENCIAS FÍSICO MATEMÁTICAS Y CIENCIAS DE LA TIERRA | |
| Aparece en las colecciones: | Articulos Arbitrados 2008 |
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| Fichero | Tamaño | Formato | |
|---|---|---|---|
| Polarization microscopy with stellated gold nanoparticles for robust, in situ monitoring of biomolecules.pdf | 1.53 MB | Adobe PDF | Visualizar/Abrir |