Please use this identifier to cite or link to this item: http://cio.repositorioinstitucional.mx/jspui/handle/1002/1007
Labeling of HeLa cells using ZrO2∶Yb3-Er3 nanoparticles with upconversion emission
TZARARA LOPEZ LUKE
Elder de la Rosa Cruz
En Embargo
01-05-2020
Atribución-NoComercial-SinDerivadas
This work reports the synthesis, structural characterization, and optical properties of ZrO2:Yb3+-Er3+ (2–1 mol%) nanocrystals. The nanoparticles were coated with 3-aminopropyl triethoxysilane (APTES) and further modified with biomolecules, such as Biotin-Anti-rabbit (mouse IgG) and rabbit antibody-AntiKi-67, through a conjugation method. The conjugation was successfully confirmed by Fourier transform infrared, zeta potential, and dynamic light scattering. The internalization of the conjugated nanoparticles in human cervical cancer (HeLa) cells was followed by two-photon confocal microscopy. The ZrO2:Yb3+-Er3+ nanocrystals exhibited strong red emission under 970-nm excitation. Moreover, the luminescence change due to the addition of APTES molecules and biomolecules on the nanocrystals was also studied. These results demonstrate that ZrO2:Yb3+-Er3+ nanocrystals can be successfully functionalized with biomolecules to develop platforms for biolabeling and bioimaging.
16-04-2015
Artículo
CIENCIAS FÍSICO MATEMÁTICAS Y CIENCIAS DE LA TIERRA
Appears in Collections:Articulos Arbitrados 2015